FastQCFastQC Report
Sun 7 Aug 2016
GBM39.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM39.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61767299
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG76150.022.492063
GTATCGA92150.019.197824
CCCGTAT128900.016.6024281
TACCGTA77400.015.4647539
GACGCTA78350.015.3322844
CAATCGG130350.014.5797444
CCGTATC132350.014.3239792
AATCGGG165300.014.1368825
ACGCTAC90600.013.4661155
TAAGGCG172550.013.3570428
CTACCGT97500.012.6617848
TCAATCG162350.012.5153183
ATCGAAG184200.012.4103436
ATCGGGA169150.012.349026
GTTCGAT93600.011.7327471
ACTACCG111200.011.4817637
GTCCGAT44150.011.4802881
CTTGGAT494900.011.0855381
CGACCGT32600.010.2211681
CTCGGAT145600.010.0566411
Produced by FastQC (version 0.11.2)