FastQCFastQC Report
Sun 7 Aug 2016
GBM39.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM39.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61767299
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG72650.020.772833
GTATCGA93650.017.470154
TACCGTA89450.016.187179
CCCGTAT128100.015.7475821
CAATCGG119150.015.4673974
CCGTATC123900.014.3812852
GACGCTA79150.014.25564
CTACCGT105750.013.9144028
TAAGGCG169750.012.8224748
ACTACCG117750.012.6959957
AATCGGG152800.012.5534175
ACGCTAC89000.012.4665765
TCAATCG149650.011.9694593
ATCGGGA159850.011.8821196
ATCGAAG194300.010.5254756
CTTGGAT476650.010.5163091
CCGTAAT75550.010.1451591
GTTCGAT86650.010.0394261
CTCGGAT146650.09.9720411
GTCCGAT42050.09.9524431
Produced by FastQC (version 0.11.2)