FastQCFastQC Report
Sun 7 Aug 2016
GBM38.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM38.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31631370
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG27000.016.1996943
CCCGTAT49100.013.0294911
GTATCGA40700.012.2489764
CCGTATC47500.011.3866512
TACCGTA34900.010.5106779
CTACCGT43650.09.8043478
CAATCGG39100.09.6228034
CTTGGAT227350.09.1452871
CTCAGAT238200.09.0446921
ATCGAAG80050.08.9891466
CTCGGTT54950.08.9029861
AATCGGG48200.08.684235
ACGCTAC39750.08.5189035
TATCGAA58950.08.4568835
CTGGAAT346350.08.3391771
TCAATCG41950.08.1842433
CCGCAAT36150.08.0677681
GTCGGTT54850.08.0616011
CCCGGAT83000.08.0478571
CTGAGAT305000.07.95829251
Produced by FastQC (version 0.11.2)