FastQCFastQC Report
Sun 7 Aug 2016
GBM28.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM28.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84873305
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG67150.011.1228323
CTCGGAT236150.010.4245131
CCCGTAT118350.010.122421
CTCCGAT172500.09.8862121
CTCGGTT157250.09.8590641
CGCGAAT32050.09.8210781
CTCAGAT644000.09.5710671
CTCGAAT193200.09.167411
CTGGAAT950150.09.1225581
CTTGGAT649250.09.0956921
CTCGCAT198550.08.9440521
GTATCGA92000.08.8843514
CTCGTAT100600.08.872951
CTCAAAT684250.08.7746231
CTCCGTT175450.08.3543691
CTGAGAT809300.08.2953581
CTCGTTT201650.08.2707051
CTCGAGT184300.08.2590881
CTGGGAT1113950.08.0679221
TACCGTA90500.08.0479669
Produced by FastQC (version 0.11.2)