FastQCFastQC Report
Sun 7 Aug 2016
GBM28.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM28.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84873305
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG65750.010.8784493
CTCGGAT230900.09.8246021
CCCGTAT117400.09.4208831
CTTGGAT638300.09.2683471
GTATCGA95200.09.2432044
CTGGAAT959550.09.1278941
CTCAGAT635650.09.1218821
TACCGTA94500.09.0622229
CTCAAAT684450.08.6571681
CTCCGAT164800.08.6532111
CCGGGAT217850.08.3175421
CGACCTA55100.08.283964
CTCGAAT198150.08.2418991
CTCGGTT157800.08.2317831
CGGGGAT223050.07.9759311
CTCGCAT196600.07.97172931
CTCGGGT208600.07.96438461
CTGAGAT824500.07.9629761
CTCGTTT192150.07.91141131
CTTGAAT634600.07.77236271
Produced by FastQC (version 0.11.2)