FastQCFastQC Report
Sun 7 Aug 2016
GBM26.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM26.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60721557
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG46350.010.4409953
CTCGGAT163550.010.2570771
CTCGTAT72200.09.8926431
CTCAGAT458850.09.8721611
CTCCGAT128950.09.5474181
CCCGTAT83100.09.2736091
CTCGGTT114550.08.9016511
CTGGAAT658900.08.8503051
CTCAAAT504500.08.7832751
CTCGACT92150.08.5668251
CTCCGGT141200.08.5527221
CTTGGAT454250.08.4928641
GTATCGA65450.08.4708464
CTCGGGT162700.08.4333721
CTCGCAT126450.08.3240591
CTGAGAT576650.08.1976931
TCAATCG69400.08.1918183
CTCGTTT132200.08.1752751
CCGCAAT71150.07.8591941
CTGGGAT842750.07.83957531
Produced by FastQC (version 0.11.2)