FastQCFastQC Report
Sun 7 Aug 2016
GBM26.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM26.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60721557
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT459900.09.344221
CTGGAAT657200.09.2104131
CGTATCG44700.09.0543943
CCCGTAT79400.08.9514251
CTCGGAT156150.08.9224771
TACCGTA54650.08.7833519
CTCGGTT106050.08.6548521
CCGGGAT170500.08.6132281
CTCCGAT120350.08.5260151
CCGGAAT124200.08.4512121
CTCGGGT159750.08.2205151
CTTGGAT441400.08.1363171
CTCGTAT80750.08.04400351
GTATCGA68000.07.95880274
CTGAGAT598400.07.87368771
CCCGGAT168750.07.8378731
CTCGAAT139900.07.73830461
CTCGTTT130000.07.67588041
CTCAAAT492750.07.60364961
CCGCAAT76000.07.5558251
Produced by FastQC (version 0.11.2)