GBM22.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	GBM22.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087784
Sequences flagged as poor quality	0
Sequence length	101
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA	6603	0.3162683495993838	No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG	4056	0.19427297076709085	No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC	3584	0.1716652680545497	No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG	3250	0.15566744452491255	No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC	2971	0.14230399313338926	No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA	2529	0.12113322067800118	No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA	2291	0.10973357397125375	No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT	2148	0.10288420641215758	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTAT	25	5.586099E-5	75.13017	1
TCGTGCG	130	0.0	57.810444	7
TCAGACG	665	0.0	51.54361	2
CCTGCCA	260	0.0	48.76237	1
CAGTTTT	860	0.0	44.7702	3
TCCCGTC	530	0.0	43.410404	2
CCGAAAG	665	0.0	42.36664	1
TGTCGAG	45	0.0010190324	41.736988	5
CACGACT	80	2.4778637E-7	41.08681	1
CCACGAG	845	0.0	40.565853	1
AGACGTG	855	0.0	40.089474	4
AGGCGTA	200	0.0	39.925335	8
TGCCGTA	565	0.0	39.890217	2
CTGCCAG	520	0.0	38.829296	1
CACGTCT	175	0.0	38.32142	94-95
TGTTACG	310	0.0	37.86622	3
CTGCCGT	635	0.0	37.712982	1
CGCTCAA	50	0.0017101305	37.565086	1
CGCCAAT	50	0.0017101305	37.565086	1
TCCGCTT	100	3.3580363E-8	37.563286	2