FastQCFastQC Report
Sun 7 Aug 2016
GBM117.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM117.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74207368
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT88650.010.9987411
CGTATCG48550.010.7719973
CTCCGAT133100.09.2902031
CTCGGAT181950.09.2317951
CTCGTAT83100.09.2266511
CTCGGTT132650.09.1880741
CCCGTCT288850.08.7304961
GTCGGTT91900.08.5360471
CTCGGGT183250.08.4649251
CTCGAAT152200.08.3864261
CTGGAAT766600.08.3771951
CTTGGAT510450.08.2918081
CTCGCAT132350.08.2042931
CGCCAAT100200.07.96166371
CTCGTTT156600.07.8677781
CCCGAAT156150.07.8336861
CCCGGAT194700.07.7167211
CTCGGCT353200.07.64181
GTCCGAT57250.07.5866431
CTCAGAT527750.07.5581121
Produced by FastQC (version 0.11.2)