FastQCFastQC Report
Sun 7 Aug 2016
GBM117.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM117.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74207368
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG23205.3547317E-415.240644-45
CGTCATA71900.014.75629448-49
ATTGCGA51606.6283974E-913.70472444-45
CGCGAAA26550.001485223513.31800246-47
TATCGCA42652.0631578E-613.027637544-45
TTCGCGA28350.002432378512.47241446-47
CGATATA44603.3967463E-612.46076548-49
TTCGCAT85200.012.45045746-47
CTTTCGC114550.012.34681544-45
TTCGCTA53401.5279875E-712.2972446-47
TGCGAAA80551.6370905E-1111.91498846-47
CGCGATT21250.011.88770348-49
CGCGTAG34307.521479E-411.78372948-49
CGAATTA51501.3123245E-611.77228848-49
GACGTAA51851.4271918E-611.69063346-47
CGCTAGT34608.091614E-411.68155948-49
GCGTATA35109.140147E-411.51299946-47
ATTTCGT106050.011.43121944-45
GCGCTAA44955.0403607E-511.23765946-47
TTCGTAA82303.0559022E-1011.04786246-47
Produced by FastQC (version 0.11.2)