FastQCFastQC Report
Sun 7 Aug 2016
GBM116.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM116.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39608215
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG49550.012.0773863
CCGACAT117200.011.6490491
ATCGAAG141500.011.4358056
CCCGTAT47850.011.1164651
CGCGTAA41150.011.0951911
TTCGCGC44550.010.7478567
GTATCGT55100.010.0574716
TATCGTT54300.09.797777
CATCGAA129350.09.5615755
GTATCGA37650.09.4192234
TACGCAC64750.09.34024450-51
TCGAAGG165250.09.3364897
ACATCGA144200.09.1917024
CCCGTCG73350.09.185671
CGACATC131450.09.1723982
TCGATCG72400.09.14767558
GACATCG145450.09.0516043
TCGCATT106000.08.9073452
CCGTTTA88650.08.85084154
CTCGGAT106600.08.7738991
Produced by FastQC (version 0.11.2)