FastQCFastQC Report
Sun 7 Aug 2016
GBM102.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM102.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103973159
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA58650.015.60375144-45
CGTATCG77600.015.13081553
TACGCGT21950.00623529713.89764744-45
TACCGTA101400.013.3442739
CGTTATA57551.9590516E-913.2531548-49
CGCATTA67501.200533E-1012.80614448-49
TGCGAAA111550.012.76233246-47
GTATCGA95550.012.5624164
CTAGCGT50359.261021E-712.11731244-45
CCCGTAT138350.012.0800911
CTAGCGA50851.0435233E-611.99816544-45
TATCGTA56502.9241164E-711.69821144-45
TTCGCTA74105.675247E-1011.66466646-47
TTCGCAT105750.011.53911446-47
TACGCTA48808.637126E-611.46078746-47
ATTTCGT130850.011.26805144-45
TAGCGAA68102.3881512E-811.19872844-45
TTAACGA96603.6379788E-1211.05265744-45
TTTCGTC143300.010.99866744-45
ATTACGC47157.672334E-510.7830844-45
Produced by FastQC (version 0.11.2)