FastQCFastQC Report
Sun 7 Aug 2016
GBM10.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM10.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458952
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC7800.024.09475
GTTACGA8500.023.2155274
TACGACT9400.020.493316
TGTTACG8800.020.2890173
TTATCCG8900.015.3094688
TATCCGG9900.014.2376459
ACGACTT14350.013.4240677
TCGTGCG10000.013.1555857
CCGACAT26100.012.9655721
CTCGCGT3651.2497681E-512.8767671
ATCGAAG28100.012.7075966
AGGCGTA13900.012.5065958
CCGGTAA8300.012.4536528
CGGTAAA8750.011.8131789
TAGCATA16950.011.6421118
GGCTCGT11850.011.4981464
GCCGTAT6201.9148501E-811.3673613
TTCGCGC7059.2040864E-1011.3295327
GACATCG30700.011.3253983
ACGAGCG6654.449248E-911.3046853
Produced by FastQC (version 0.11.2)