FastQCFastQC Report
Sun 7 Aug 2016
GBM85.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM85.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85402425
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT94550.010.7479741
CGTATCG79250.010.4687343
GTATCGA75400.09.347894
ATCGAAG196100.09.247686
CCGTATC117450.08.4390922
CTCGCAT172350.08.288951
CTCGGAT179550.08.0385871
CCGGTAT140700.07.9291681
CCCGGAT215300.07.8667051
TGCGCGA72950.07.85024559
CGCGAAT25400.07.82778931
CGGTATT132750.07.6600252
CTCCGAT112250.07.6427321
CTCGGGT218400.07.51902061
CGGGAAT145100.07.5110971
TATCGAA100800.07.5022265
CTCGGTT112350.07.43929621
CTGGAAT736800.07.35592461
CGACCTA39150.07.3138584
TCGAAGG244150.07.3074027
Produced by FastQC (version 0.11.2)