FastQCFastQC Report
Sun 7 Aug 2016
GBM84.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM84.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254234
Sequences flagged as poor quality0
Sequence length83
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA41830.18556192480461212No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCNA40270.1786416139584444No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG25070.11121294417527196No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATNG24840.11019264193513184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCC150.001857780473.258163
GCGTATC150.001857780473.258164
CGTGGTT251.8812461E-458.7866331
TTACGTT251.9099985E-458.6065372
TACGTTC251.9099985E-458.6065373
GTTAGGA1150.057.5086631
CCTCGAC200.00573775955.112471
CCGCGAT200.00573775955.112471
GCTCGAC200.00573775955.112471
CCTGCCA2300.052.7162741
CACGACT351.566651E-552.4880681
CTTATAG304.628456E-448.9888571
CGCGTTC304.6990332E-448.838783
GTAGTAT304.6990332E-448.838788
TACATCG559.183714E-846.6188353
CGTGGTA656.712071E-945.0819473
TGCCGTA3600.044.7688832
CGTCGCA601.7766251E-742.865251
TCAGACG9350.042.3095322
CTCGCTA350.001004183841.8618132
Produced by FastQC (version 0.11.2)