FastQCFastQC Report
Sun 7 Aug 2016
GBM81.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM81.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552603
Sequences flagged as poor quality0
Sequence length83
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG16350.018.1911833
CCCGTAT27700.014.7144081
GTATCGA22250.013.3673814
CCGTATC28250.012.9977152
TACCGTA17900.012.1044969
GACGCTA21500.011.2718934
CTCCGAT32550.011.0554061
GATCGGA16500.010.4595621
ACGCTAC25400.09.6862035
CCCGGTT20700.09.57906151
CTACCGT23800.09.5667088
TCCGATT27400.09.380652
CCGGTCG18900.09.3255213
CCCGGAT37250.09.2661891
ATCGAAG41550.09.1030496
CCGGTAA14500.08.8633851
CGCGAAT5853.05504E-68.787631
CCCCGGT31000.08.6469061
CGACCTA8501.5988917E-98.6397754
CTCGTTT30450.08.3207291
Produced by FastQC (version 0.11.2)