FastQCFastQC Report
Sun 7 Aug 2016
GBM76.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM76.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58615805
Sequences flagged as poor quality0
Sequence length83
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT57900.010.6640111
CGTATCG42000.010.0570693
CTCCGAT92400.09.2677391
CTCGCAT122850.08.975031
CTCGGAT124950.08.8830181
ATCGAAG138100.08.8037116
CGCGTAA29500.08.5964061
CTCGGTT82950.08.595581
TCGATCG58500.08.4781238
TCGCATT94200.08.3827312
CCGACAT127500.08.3017981
CCGGTAT91250.08.1359511
GTATCGA42800.08.0670234
CCGTATC74400.07.8491422
CCCGGTT86000.07.82064341
CTCAGAT340500.07.79309371
GTCCGAT39800.07.75686171
CGCGAAT13300.07.7374211
CTCCGGT110400.07.6901141
CTGGAAT493300.07.61429451
Produced by FastQC (version 0.11.2)