FastQCFastQC Report
Sun 7 Aug 2016
GBM59.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM59.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99372724
Sequences flagged as poor quality0
Sequence length83
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG84250.018.4869163
CCCGTAT145800.015.756051
GTATCGA111200.014.86572554
CCGTATC145900.013.3684952
TACCGTA92850.011.3572599
TAAGGCG179600.010.5768798
CAATCGG107550.09.9052624
CTACCGT108650.09.84093958
CTCCGAT191050.09.8117851
CTTGGAT622150.09.5411841
ATCGAAG229550.09.345826
CTCGGAT197000.09.1609421
AATCGGG129900.09.0779565
CTGGAAT831350.08.93084051
ACTACCG131900.08.6062027
CTCAGAT619750.08.5402551
CTCGGTT131500.08.4971381
CTCGAAT185200.08.4943011
CGACCGT45650.08.37371
TATCGAA216050.08.3317075
Produced by FastQC (version 0.11.2)