FastQCFastQC Report
Sun 7 Aug 2016
GBM59.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM59.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99372724
Sequences flagged as poor quality0
Sequence length83
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG83750.017.3121643
GTATCGA112300.014.6105154
CCCGTAT148350.014.3721871
CCGTATC138750.013.2009812
TACCGTA97200.011.3667259
TAAGGCG176450.010.4012228
CTACCGT112700.010.29198558
CTTGGAT607200.09.6487321
CTCGGAT192350.09.24671
ATCGAAG232600.09.1212786
CTCCGAT185150.09.0295061
ACTACCG136000.08.9605617
CGGGGAT186550.08.9420021
CTCGGTT130150.08.8275921
CAATCGG101250.08.7732014
CTGGAAT831350.08.5709491
TATCGAA216100.08.441885
CGGGGTT224300.08.307171
AATCGGG127950.08.2620875
CTCAGAT605500.08.2223211
Produced by FastQC (version 0.11.2)