FastQCFastQC Report
Sun 7 Aug 2016
GBM5.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM5.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80447447
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG61200.011.061463
CTCGTAT80900.010.2715341
CCCGTAT95300.010.0516311
CTCGGAT182950.09.7990421
GTATCGA76900.09.75260454
CTCGGTT116400.09.0558171
CTTGGAT474250.08.8744211
CTGGAAT721050.08.8460461
CTCGAAT150250.08.4238671
TACCGTA59450.08.42120559
CAATCGG82600.08.3346114
CGGAGAT162800.08.1053131
CTCGGGT194250.08.0605281
CCGGGAT180150.08.0507651
CCGTATC105500.08.0208642
CTCCGAT142350.07.91845131
CTCAGAT506750.07.88772731
TCAATCG81200.07.81590183
CTCGACT109050.07.79644541
TAAGGCG123600.07.78945838
Produced by FastQC (version 0.11.2)