FastQCFastQC Report
Sun 7 Aug 2016
GBM5.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM5.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80447447
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA60450.011.1394089
CGTATCG59600.011.0402763
CCCGTAT101050.010.2464891
CTCGGAT181900.09.3529521
CTTGGAT482250.08.9632731
CTCGTAT90050.08.8480131
CTACCGT85350.08.7461838
GACGCTA67700.08.3550494
CCGTATC99700.08.3371692
GTATCGA82050.08.3007064
CCGGGAT181550.08.2473131
CTGGAAT717700.08.1680061
CAATCGG81900.07.89306554
CTCGGTT112150.07.7908651
CGGGTAT56300.07.6571781
CTCAGAT508600.07.4772121
CTCAAAT533600.07.45871161
CGAAGAT218900.07.4379581
CTCGGGT185800.07.41645571
ACTACCG99350.07.39751437
Produced by FastQC (version 0.11.2)