FastQCFastQC Report
Sun 7 Aug 2016
GBM44.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM44.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699621
Sequences flagged as poor quality0
Sequence length83
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1500.046.1121941
TCGGAAG1500.040.9856873
AGAGCGT851.020453E-940.7338268
GTACCTT400.001529661638.424083
AAGAGCG1350.037.0459867
GACCGGA450.002726858234.1547432
TCGGGAA907.74271E-834.1547433
TCGAATC450.002726858234.1547432
CGGAAGA1750.032.932574
ATCGGGA1305.638867E-1132.5126882
TTATCCG2050.031.8615954
AGAGCGG1101.2681994E-831.4761358
AGACGGC500.00454121930.7766699
CTTCGTT500.004566719830.7414651
TATCCGG2150.030.3753195
CGTATCG2450.029.7982673
CCTCACC7100.029.7672621
CGAATCT654.6092615E-429.5569883
TCCGGTA2300.028.4349657
AAGCGGC954.504245E-628.3469319
Produced by FastQC (version 0.11.2)