FastQCFastQC Report
Sun 7 Aug 2016
GBM40.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM40.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35270278
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT38800.011.1081291
CGTATCG30200.010.9559513
GTATCGA32550.010.2827134
CCCGGTT56200.08.216741
CTCCGAT64600.08.1014181
TACCGTA36300.07.8425489
CGACCGT25100.07.512371
CTCGGTT48650.07.43531941
TCGCATT44750.07.3940192
CCGTATC48000.07.37431572
CTACCGT39150.07.36989978
CTCGCAT70750.07.3428041
ATCGAAG79000.07.15850456
CGGGGTT97300.07.15847251
CCCGGAT91550.07.1457071
CCGGGAT90600.06.83836561
CTCGGAT71750.06.8114011
CCGGTAT45200.06.7257891
CCCGAAT78800.06.6415171
GTCCGAT23800.06.62920571
Produced by FastQC (version 0.11.2)