FastQCFastQC Report
Sun 7 Aug 2016
GBM39.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM39.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61767299
Sequences flagged as poor quality0
Sequence length83
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG65600.021.409863
GTATCGA79500.018.2473034
CCCGTAT104850.016.7368871
TACCGTA67450.014.55192859
CAATCGG109750.014.1995624
CCGTATC110700.014.0429562
GACGCTA70950.013.8839174
AATCGGG138300.013.855525
CTACCGT82200.012.3153348
TAAGGCG153950.012.2762038
ATCGAAG153550.012.2079346
ACGCTAC82350.012.1485775
TCAATCG142150.011.7210313
ATCGGGA147900.011.5812336
ACTACCG91650.011.4235077
GTCCGAT37650.011.0391851
GTTCGAT83200.010.8235771
CTTGGAT418500.010.7497241
CGACCGT27000.010.1198221
TATCGAA173900.09.8684955
Produced by FastQC (version 0.11.2)