FastQCFastQC Report
Sun 7 Aug 2016
GBM39.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM39.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61767299
Sequences flagged as poor quality0
Sequence length83
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG62750.019.6881453
GTATCGA79200.016.9108924
CCCGTAT102150.016.1663481
TACCGTA77200.015.353999
CCGTATC103800.014.0526712
CAATCGG107900.013.9822514
CTACCGT90450.013.3175288
GACGCTA71350.012.9458254
ACTACCG101250.012.0870417
AATCGGG132700.011.8331865
TAAGGCG152400.011.6918688
ACGCTAC80700.011.255145
ATCGGGA141550.010.9845936
TCAATCG136850.010.7150383
ATCGAAG162700.010.2899716
CTTGGAT408450.010.0464191
CCGTAAT63700.09.8501121
GTCCGAT35600.09.6234991
CCGCAAT55100.09.4313781
CTCGGAT127550.09.3858351
Produced by FastQC (version 0.11.2)