FastQCFastQC Report
Sun 7 Aug 2016
GBM38.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM38.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31631370
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG23850.015.0082313
CCCGTAT39950.013.1050731
GTATCGA34900.011.6900424
CCGTATC40450.010.9425462
TACCGTA30050.09.989859
CTACCGT37100.09.4400948
CAATCGG35000.08.7974654
CTTGGAT193550.08.7911641
CTCAGAT202400.08.7110861
ATCGAAG67650.08.7048096
CTCGGTT46150.08.675191
TATCGAA49100.08.3092155
CTGGAAT285700.08.2732361
AATCGGG41700.08.214665
ACGCTAC34100.08.126695
CCGCAAT29450.08.1044531
CTCCGAT50900.08.0168811
CGCGAAT6851.3489429E-57.86782651
CGCGTAA7851.2309047E-67.84634971
CCCGGAT70000.07.80921941
Produced by FastQC (version 0.11.2)