FastQCFastQC Report
Sun 7 Aug 2016
GBM34.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM34.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78361977
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT87500.010.6868841
CTCGTAT64800.09.7391641
CGTATCG54350.09.698943
CTCGGAT175050.09.6945881
CTCGGTT123600.08.966581
CTGGAAT721900.08.8807741
CTCGCAT131600.08.8016371
CTCCGAT132900.08.6286751
CTCAGAT513400.08.5897711
GTATCGA73950.08.4290784
CTTGGAT501400.08.411611
CTCAAAT507000.08.1668991
CCGGGAT186950.08.0071331
CGCCAAT92150.07.97610571
CTCGAAT135800.07.96268221
CTCCGGT147050.07.92921921
CGCGAAT19450.07.9139491
CTCGTTT139700.07.85057261
CTCGGGT167150.07.82754231
CTGAGAT624900.07.66059831
Produced by FastQC (version 0.11.2)