FastQCFastQC Report
Sun 7 Aug 2016
GBM34.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM34.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78361977
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG51350.010.2678413
CTCGGAT172450.09.7989331
CCCGTAT85000.09.7816541
CTCGGTT119000.08.92771
CTGGAAT706600.08.5091241
CTCAGAT509050.08.2497681
CTTGGAT488350.08.2447591
CTCCGAT132150.08.1849611
GTATCGA69150.08.1813144
CTCGTTT132900.07.8780981
CTCAAAT492800.07.8110021
CCCGGAT184200.07.7737541
CTCGCAT136250.07.71264931
CCGGAAT121450.07.70169351
CTCGAAT132050.07.66645861
CCCGAAT151800.07.4804491
CTCGAAC188000.07.4733011
CGCCAAT90850.07.4146491
CTGAAAT672300.07.3572831
TCGGATT96100.07.1284952
Produced by FastQC (version 0.11.2)