FastQCFastQC Report
Sun 7 Aug 2016
GBM26.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM26.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60721557
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT58100.010.06714251
CGTATCG39850.09.9448023
CTCGGAT138900.09.8901921
CCCGTAT66250.09.5256971
CTCCGAT105850.09.5246531
CTCAGAT390250.09.5054541
CTGGAAT546950.08.7309781
CTCGGTT95850.08.7117691
CTCCGGT114750.08.6182531
CTCAAAT424550.08.5471551
CTCGCAT103300.08.34423351
CTCGACT77750.08.314721
CTTGGAT381500.08.2810881
CTCGGGT136150.08.2528511
GTATCGA55100.08.2398194
CTCGTTT110050.08.0422321
TCAATCG58150.08.0061353
CCGCAAT58000.07.89510151
CTGAGAT491900.07.86973141
CTCCAAT316950.07.6608571
Produced by FastQC (version 0.11.2)