FastQCFastQC Report
Sun 7 Aug 2016
GBM26.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM26.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60721557
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT388700.09.0415241
CTGGAAT548650.09.0225851
CCCGTAT64750.08.9768221
CGTATCG38200.08.6646943
TACCGTA45500.08.6275749
CTCGGAT133350.08.544451
CTCGGTT89150.08.419741
CCGGAAT102300.08.3910171
CTCCGAT100250.08.3706181
CCGGGAT145550.08.2514031
CTCGTAT64650.08.2166711
CTCGGGT135250.07.9405821
CTTGGAT375750.07.8164751
GTATCGA57100.07.7512234
CCGCAAT60950.07.70498041
CTGAGAT501500.07.68331341
CCCGGAT141800.07.6280841
CTCGAAT116450.07.60280281
CTCGTTT108000.07.55609661
CGATAAC49400.07.48048351
Produced by FastQC (version 0.11.2)