FastQCFastQC Report
Sun 7 Aug 2016
GBM22.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM22.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087784
Sequences flagged as poor quality0
Sequence length83
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA66030.3162683495993838No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG40560.19427297076709085No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC35840.1716652680545497No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG32500.15566744452491255No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC29710.14230399313338926No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA25290.12113322067800118No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA22910.10973357397125375No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT21480.10288420641215758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT251.5016699E-461.527681
TCGTGCG1250.049.2374767
CCTGCCA2350.044.182111
CGCCAAT357.903544E-443.948351
TCAGACG6550.042.8559722
CACGACT652.3287976E-741.4128651
CAGTTTT7900.039.91323
TGTCGAG400.001524719138.452965
CCGAAAG6250.036.916611
TCCCGTC5200.036.234522
CCGGCAT854.658068E-836.1927571
TCCGCTT854.660069E-836.191022
CCACGAG8150.034.444181
AGGCGTA1900.034.417658
CGCTCAA450.002717424634.182051
CTGCCAG4850.034.0939521
GTTAGGA2150.033.983311
TGCCGTA5500.033.558952
AGACGTG8500.033.0243074
CTGCCGT6100.032.1507381
Produced by FastQC (version 0.11.2)