FastQCFastQC Report
Sun 7 Aug 2016
GBM16.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM16.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20091558
Sequences flagged as poor quality0
Sequence length83
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26150.012.07181551
GTATCGA19550.010.4366114
GTCTAAC30500.010.0976554
CTCGTAT16050.010.0740631
CGTATCG27250.09.8892173
ATCGAAG61800.09.5932786
GCGAACG9253.6379788E-119.1561173
CTCCGAT33850.08.9846011
CGACCTA10202.910383E-118.6807664
GTTACGG37050.08.31259257
AGTTACG42150.08.1288146
CCGACAT48000.07.94007061
CCGTATC37700.07.7607312
TCGAAGG80100.07.73798857
TCCGATT27400.07.7275632
TTATCCG30400.07.72484738
TCGCATT36550.07.68892962
CGATTAG28050.07.68573764
CGCATTC41450.07.33724453
CTCGCAT52650.07.311931
Produced by FastQC (version 0.11.2)