FastQCFastQC Report
Sun 7 Aug 2016
GBM155.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM155.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35009954
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT37150.010.3597921
CGTATCG29600.09.2292433
CCCGGAT96800.08.0710591
CCGTATC46150.07.67078452
CTCCGAT57650.07.6105391
CGGGGTT94150.07.31716061
CGCTAAT16000.07.2162431
CCCGGTT53950.07.20509621
CTCGCAT77300.07.16956571
GTATCGA31800.07.13877534
CGCCAAT43150.06.95702651
ATCGAAG87150.06.9315226
CCGGTAT68400.06.86457441
TCGCATT51700.06.8473252
CCGCAAT35150.06.7885381
CTCGAAT63000.06.71988731
CCGGGAT99900.06.62632661
CTCGGTT47250.06.59770771
CTCGGAT74350.06.522281
AGTTACG52400.06.46171436
Produced by FastQC (version 0.11.2)