FastQCFastQC Report
Sun 7 Aug 2016
GBM150.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM150.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35614525
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT44950.09.9603061
CGACCTA18200.09.7334024
TGCGCGA42300.08.466339
CGTATCG30300.08.3883263
CCGCAAT36650.08.2141941
CGGGGTT92450.08.1826411
CTCGCGT23850.07.7677851
CCGGTAT69050.07.43416741
CTCGTAT28100.07.417061
CCGGGAT99750.07.3129611
CCGGAAT60550.07.26666361
CCCGGAT107350.07.26262571
CACGGAT70300.07.1921761
TCGCATT60200.07.1644672
GTTCGAT59450.06.94666341
CTCGCAT73550.06.9268471
CTCGAAT56450.06.90560531
CCGTATC57350.06.84903052
CTCCGGT72150.06.79378371
GTCCGTT28450.06.783161
Produced by FastQC (version 0.11.2)