FastQCFastQC Report
Sun 7 Aug 2016
GBM150.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM150.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35614525
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT41550.09.2904361
TGCGCGA47650.08.724069
CCGGGAT96400.08.329011
CGCGATT9051.5690784E-77.6776981
CTCCGAT64250.07.57015131
CGTATCG32650.07.5626513
CCGGAAT59850.07.4172141
CCCGGAT101250.07.24378631
ATCGAAG96850.07.23329646
TCGCATT60700.07.0552162
CGGGGTT85800.07.0185021
CCGGTAT72100.07.01363471
GTCTAAC51900.06.99145561
CTCGCAT77500.06.92341231
CTCGAAT56500.06.9004911
CTCGGAT73950.06.83817531
CGCGTAA10854.4250191E-76.75975471
CGCGTTT13752.1518645E-96.73776151
CGCGAAT9754.1274507E-66.7305631
ACGTGCG76800.06.715966
Produced by FastQC (version 0.11.2)