FastQCFastQC Report
Sun 7 Aug 2016
GBM15.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM15.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53242722
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG32700.010.8281773
CCCGTAT58100.010.3356941
CTCGGAT119600.09.1728621
CGCGTAT14550.08.9951231
CTGGAAT494800.08.9310671
CTCGCAT86750.08.69716551
CTCGGTT81750.08.6169681
TACCGTA40100.08.5416669
CCGGGAT125150.08.3354611
CTCCGAT89900.08.1355161
CTCAGAT342300.08.0743721
CTTGGAT344750.08.0728191
GTATCGA51400.08.0117354
CTCGGGT112950.07.9748181
CTCGTTT95350.07.75124931
CTCGAAT101650.07.7252751
CCCGGAT121500.07.60372071
CCGTATC62300.07.5990862
CCGGAAT88850.07.58179041
CGATAAC44700.07.57820461
Produced by FastQC (version 0.11.2)