FastQCFastQC Report
Sun 7 Aug 2016
GBM148.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM148.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70581861
Sequences flagged as poor quality0
Sequence length83
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG68250.012.9089733
CCCGTAT89150.012.5179891
GTATCGA75900.011.5059794
CCGTATC105350.09.4223142
CGCGAAT19800.08.3572071
CGGGGAT217000.07.8205411
CCGGGAT205050.07.7883641
CGGGGTT207850.07.6649311
ATCGAAG196800.07.52600156
GTCCGAT63300.07.477551
TATCGAA121650.07.3683885
CCCGGAT216650.07.3003061
CTCGGAT162450.07.29606871
CCCGGTT114250.07.20801161
CGACTAA81450.07.06366428-29
CCGGTAT106750.06.9574061
CGACCGT57750.06.9300961
TCGCATT97100.06.854872
CTTGGAT408200.06.75933651
GTCCGTT56700.06.6512141
Produced by FastQC (version 0.11.2)