FastQCFastQC Report
Sun 7 Aug 2016
GBM14.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM14.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33448665
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG35400.010.6511123
TCGATCG41400.010.1339528
CTCAGAT204400.010.052651
CTCGCAT79000.010.0336811
CCCGTAT41750.09.8616031
CTCGGTT53950.09.6999091
CGCGAAT10000.09.6196711
CTCGGAT75050.09.5876261
CTCGTAT31650.09.4828991
CGATCGG43600.09.4460449
CTTGGAT213350.09.2702331
CTCCGAT58050.09.0811011
CCGTACG37900.08.9333754
CTGGAAT314000.08.7863741
ATCGAAG102500.08.749536
TGCGCGA26050.08.7176019
TCGCATT62400.08.6320832
GTATCGA32750.08.5759794
CTCGAAT63800.08.503911
CGTACGC41700.08.1191345
Produced by FastQC (version 0.11.2)