FastQCFastQC Report
Sun 7 Aug 2016
GBM134.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM134.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43274219
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG46900.012.3383743
CCCGTAT56550.011.7395021
GTATCGA47350.011.2433474
CTACCGT43600.09.358068
CCGTATC74700.08.8310432
TACCGTA48200.08.3052519
ATCGAAG114350.08.0115546
TATCGAA76050.07.79474645
GTCTAAC64300.07.3795644
ACTACCG56300.07.1103577
TCGCATT62100.07.0197782
CTCCGAT76100.06.89776041
CGACTAA44500.06.83332428-29
TCGAAGG132200.06.8131957
GTCGAAT27750.06.67624241
CGCGAAT11106.578284E-76.6066981
CCGGGAT124050.06.59618661
CGTCGAT29700.06.4978161
CCCGGTT69950.06.45582871
CCGCAAT39600.06.43283751
Produced by FastQC (version 0.11.2)