FastQCFastQC Report
Sun 7 Aug 2016
GBM123.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM123.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46065893
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT64050.010.3034481
CGACCTA21450.010.2333524
CGTATCG45100.010.1609233
CCCGGAT131450.09.4830381
GTATCGA49400.08.5750384
CTCCGAT81800.08.1148541
CTCCGGT79800.07.7378921
GTCCGAT30650.07.68077331
CCGCAAT46250.07.67682551
ATCGAAG109850.07.64250476
GCGAACG29350.07.60996533
CCGTATC80100.07.5958522
CGCGATT13907.2759576E-127.4964311
GTCCGTT37600.07.4927391
CCCGGTT68500.07.4368491
CCCGAAT131100.07.1827831
TCCGATT66200.07.15482572
CTCGGAT115800.07.1319911
CGCCAAT65450.07.0758261
CCGGGAT116700.07.04391861
Produced by FastQC (version 0.11.2)