FastQCFastQC Report
Sun 7 Aug 2016
GBM123.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM123.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46065893
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG73450.012.73419744-45
TATGCCG81300.011.71760748-49
CGTATGC83900.011.53798146-47
CCCGTAT60300.011.137691
CGTATCG48800.010.1187963
CTCGTAT89900.010.10437444-45
CGACCTA24400.09.48634054
GTATCGA51450.09.447624
CCGTATC65300.09.275182
GATCGGA206500.08.485921
CGCGTAT10454.2382453E-108.1258641
ATCGGAA219700.08.0596922
GCGAACG27950.08.0054343
TCTCGTA124350.07.552672442-43
CCCGGAT132550.07.54194261
CGACCGT32100.07.4550371
ATGCCGT137400.07.423579748-49
TCGGAAG250500.07.4229743
CTCGGAT120150.07.1959381
CGGGGTT142850.07.13323971
Produced by FastQC (version 0.11.2)