FastQCFastQC Report
Sun 7 Aug 2016
GBM120.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM120.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25792405
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT31400.013.9710081
CGTATCG27250.012.9892413
GTATCGA25950.012.6015694
CCGTATC39150.010.1223112
ATCGAAG66250.09.0007346
TATCGAA38900.08.9007185
CGCGTAT5153.2980967E-48.2193591
TACCGTA27850.08.01188859
CTACCGT27050.07.96439658
CTCCGAT43550.07.9525581
CCCGGAT77550.07.8898341
CGGGGTT76750.07.82165671
TCGAAGG77350.07.7588337
CCCGGTT39000.07.69630861
CGCTAAT10503.4098775E-87.3298181
CGCGGAT25500.06.9417691
GTCGGAT24650.06.86891751
TCGCATT37800.06.81957342
CCGGAAT42600.06.77492051
CCGGTAT49600.06.74978731
Produced by FastQC (version 0.11.2)