FastQCFastQC Report
Sun 7 Aug 2016
GBM12.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM12.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635321
Sequences flagged as poor quality0
Sequence length83
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG1150.046.823277
AGACGGC2150.046.5121549
CCGGAAA851.0404619E-940.6570784
GACCGGA802.757588E-838.4044042
AATCGGA509.901992E-538.4044042
GCTCGTG1400.038.401385
GGCTCGT1600.035.9984554
ATCGGGA1202.1827873E-1135.2040372
TGTTACG1750.035.11263
TCGGAAA1650.034.9075934
GAATCGG450.002728251534.15071
TTACGAC1800.034.1345565
AGGCGTA1500.033.333718
CATACGA950.032.34564614-15
GTTACGA1900.032.3354574
CGGAAAG2050.031.8450475
ATACGAG1000.030.7671516-17
GATCGGA1401.3460522E-1030.1748892
TCGGAGA902.9996754E-629.8653854
ACCGGAA654.6226324E-429.541853
Produced by FastQC (version 0.11.2)