FastQCFastQC Report
Sun 7 Aug 2016
GBM117.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM117.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74207368
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG18805.650533E-415.13304844-45
CGTCATA58050.014.70612248-49
ATTGCGA42208.3291525E-913.48347544-45
CGCGAAA21400.001504808713.29486846-47
TATCGCA35503.0165247E-612.59361944-45
TTCGCAT68650.012.43307546-47
CGATATA36203.744035E-612.35279548-49
CTTTCGC92250.012.33610144-45
TGCGAAA64351.4551915E-1112.00065246-47
TTCGCTA44052.1121195E-711.99491746-47
GCGTATA27256.7704974E-411.93227546-47
CGCTAGT27657.6396595E-411.76185848-49
CGAATTA41551.3556364E-611.74062748-49
CGCGATT17351.8189894E-1211.71525248-49
CGCGTAG27807.994446E-411.69839548-49
TTCGCGA24350.003965772711.68419646-47
GACGTAA42251.6616468E-611.54394646-47
ATTTCGT84650.011.52313144-45
GCGCTAA35954.7392343E-511.30578946-47
TTTCGTC84250.011.09543144-45
Produced by FastQC (version 0.11.2)