FastQCFastQC Report
Sun 7 Aug 2016
GBM116.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM116.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39608215
Sequences flagged as poor quality0
Sequence length83
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG10957.05586E-933.43522644-45
CGCGATT10151.3496538E-732.06784448-49
CGATCGT8956.691398E-527.27119444-45
TTCGCGA15254.7109424E-621.34070846-47
TTGCGAC19901.0063741E-720.44197744-45
TCGCGAT18159.406558E-720.1722646-47
TTAGCGC17901.8797697E-518.18079644-45
GCGCGAA13950.001343301517.49708646-47
CGCTAGT14200.001511701417.191348-49
TCCGTAT39109.094947E-1216.64684346-47
TTCGCGT14750.001951410216.54760644-45
CGCGTAT7452.1606993E-616.38365648-49
TAGTCGA14950.002135179216.32623344-45
CGAATTA20054.9692786E-516.23384748-49
GTTGCGA20054.9761285E-516.23123644-45
GTCGTAA25251.2245018E-616.11117746-47
ATCGCAT25451.3295394E-615.98456746-47
CTATCGT18054.1180514E-415.77599644-45
CACGCGA15750.003023274515.49696544-45
TATCCGT23651.1747339E-515.48058344-45
Produced by FastQC (version 0.11.2)