FastQCFastQC Report
Sun 7 Aug 2016
GBM115.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM115.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83611292
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG80050.010.8240083
CCCGTAT109900.010.6054671
GTATCGA80950.09.7523124
GTCCGAT57600.08.4424521
CCGTATC138600.07.66835832
ATCGAAG206450.07.62938746
CTCCGAT140250.07.3748381
CCCGGAT236950.07.2480941
CGCGTAA21500.07.1802911
GTCCGTT73650.07.17907331
CTCGGAT190950.07.17512131
CCCGGTT134550.07.11358451
CCGGTAT126550.07.10582161
CCGGGAT240300.07.0185631
CCGGAAT138300.06.94860651
CGGGGTT219250.06.93549061
CCCGAAT178600.06.80690431
CGCGAAT23900.06.78222131
CTCGTAT72300.06.6725571
CGGGGAT245100.06.5661881
Produced by FastQC (version 0.11.2)