FastQCFastQC Report
Sun 7 Aug 2016
GBM110.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM110.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11583822
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA11300.016.3507794
TTACGAC11150.016.2257985
TACGACT13050.014.1634646
CGTATCG10650.013.7343973
TGTTACG13550.013.3516263
TCACCCG34050.012.0960133
AGTACGC17200.011.41429748-49
CCCGTAT10850.011.0034491
TACGCAC19000.010.83945950-51
CCCGGTT16400.010.0976771
CTCGTAT10000.010.0131381
TTATCCG15800.09.99238
ACGACTT21000.09.9017797
CCCGTCG24150.09.5682161
CACCCGG43800.09.491294
ATTCCGT26850.09.46177152-53
ATCGAAG34350.09.4165396
GCGGTAT15650.09.3464113
CTCGATT29150.09.11208948-49
TAGCATA31650.08.7601329
Produced by FastQC (version 0.11.2)