FastQCFastQC Report
Sun 7 Aug 2016
GBM108.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM108.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60267933
Sequences flagged as poor quality0
Sequence length83
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG34750.014.0949993
CCCGTAT65550.010.7136311
GTATCGA47850.010.6878914
CTCGGAT115700.09.556081
CTCCGAT93000.09.3714581
CTCGTAT51250.08.9245151
CTCGCAT89150.08.4834541
CCCGTCT192150.08.3030581
CTGGAAT496500.08.2256171
CTCAGAT362750.08.1410651
ATCGAAG110400.08.09110456
CTCGTTT93900.08.0159581
GTCGAAT39650.07.9930811
CTTGGAT335450.07.9876661
CCGTATC72300.07.92015932
CCCGGAT134500.07.73838471
CGGGAAT92800.07.68408161
TATCGAA73050.07.592535
CTCGAAT99700.07.58575631
CGATAAC49300.07.52428151
Produced by FastQC (version 0.11.2)