FastQCFastQC Report
Sun 7 Aug 2016
GBM102.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM102.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103973159
Sequences flagged as poor quality0
Sequence length83
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG72800.013.0104563
CCCGTAT112150.012.0417971
GTATCGA85300.011.3995334
TACCGTA82400.09.8387299
CTCGGAT195850.09.819221
CTCCGAT186400.09.5055771
CTCGGTT161150.09.40829851
CTCGCAT179750.09.2161231
CTTGGAT611550.09.0157641
CTCGTAT91650.08.9983381
CCGTATC135550.08.9800462
CTCAGAT644550.08.8949961
CTCGAAT186900.08.5745241
CTGGAAT868000.08.5551611
CTCGTTT177750.08.48914051
CTCAAAT648650.08.28912451
ATCGAAG203500.08.1947336
TAAGGCG148150.08.1840768
AATCGGG115300.08.183615
CCCGTCT364550.08.169731
Produced by FastQC (version 0.11.2)