FastQCFastQC Report
Sun 7 Aug 2016
GBM10.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM10.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458952
Sequences flagged as poor quality0
Sequence length83
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC6950.022.132175
TGTTACG7000.021.4264983
GTTACGA6850.021.3321134
TACGACT8400.019.2438966
TTATCCG7950.018.8896398
CGTATCG6900.016.720793
TATCCGG9000.016.2580059
TTCGCGC8850.015.2282847
TCACCCG27350.014.6238143
CCGTATC9250.013.7200862
ACGACTT11400.013.5108087
GCCGTAT6650.013.30363751
AGGCGTA10400.012.5884368
CCCGTAT5604.1836756E-1112.3637081
CACCCGG31650.012.2712414
TATCGTT7600.011.637595
CCGGCAT9950.011.5974471
TCGTGCG8000.011.5517427
GTGCGTC7001.8189894E-1211.5517419
GTATCGT8200.011.2548124
Produced by FastQC (version 0.11.2)